Impression of Preanalytical Components on the Measurement of Tumor Tissue Biomarkers Utilizing Immunohistochemistry
Evaluation of formalin-fixed paraffin-embedded (FFPE) tissue by immunohistochemistry (IHC) is commonplace in scientific and analysis laboratories. Nonetheless, reviews recommend that IHC outcomes might be compromised by biospecimen preanalytical elements. The Nationwide Most cancers Institute’s Biospecimen Preanalytical Variables Program performed a scientific research to look at the potential results of delay to fixation (DTF) and time in fixative (TIF) on IHC utilizing 24 most cancers biomarkers. Variations in IHC staining, relative to controls with a DTF of 1 hr, had been noticed in FFPE kidney tumor specimens after a DTF of ≥2 hr.
Reductions in H-score and/or staining depth had been noticed for c-MET, p53, PAX2, PAX8, pAKT, and survivin, whereas will increase had been noticed for RCC1, EGFR, and CD10. Extended TIF of 72 hr resulted in considerably lowered H-scores of CD44 and c-Met in kidney tumor specimens, in contrast with controls with 12-hr TIF. An elevated likelihood of altered staining depth attributable to DTF was noticed for 9 antigens, whereas for extended TIF an elevated likelihood was noticed for one antigen.
Outcomes reported right here and elsewhere throughout tumor sorts and antigens assist limiting DTF to ≤1 hr when attainable and fixing tissues in formalin for 12-24 hr to keep away from confounding results of those preanalytical elements on IHC. The persistent an infection of high-risk human papillomavirus (HR-HPV) is among the most typical causes of cervical most cancers worldwide, and HPV kind 58 (HPV58) is the third most typical HPV kind in japanese Asia. The E7 oncoprotein is constitutively expressed in HPV58-associated cervical most cancers cells and performs a key function throughout tumorigenesis. This research aimed to evaluate the HPV58 E7 protein expression within the tissues of cervical most cancers and cervical intraepithelial neoplasia (CIN).
NKX3.1 immunohistochemistry is very particular for the prognosis of mesenchymal chondrosarcomas: expertise within the Australian inhabitants
![]() Immunohistochemistry Kit (OKRA00037) |
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OKRA00037 | Aviva Systems Biology | 1 kit | EUR 805 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
![]() Immunohistochemistry Kit (OKRA00038) |
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OKRA00038 | Aviva Systems Biology | 1 kit | EUR 805 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
![]() Immunohistochemistry Kit (OKRA00039) |
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OKRA00039 | Aviva Systems Biology | 1 kit | EUR 805 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
![]() Immunohistochemistry Kit (OKRA00040) |
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OKRA00040 | Aviva Systems Biology | 1 kit | EUR 805 |
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
![]() BrdU/ Rat BrdU ELISA Kit |
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ELA-E1336r | Lifescience Market | 96 Tests | EUR 886 |
![]() Brdu |
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20-abx082348 | Abbexa |
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![]() BRDU antibody |
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20R-BS001 | Fitzgerald | 250 ug | EUR 553 |
Description: Sheep polyclonal BRDU antibody |
![]() BRDU antibody |
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10R-2321 | Fitzgerald | 1 mL | EUR 601 |
Description: Mouse monoclonal BRDU antibody |
![]() BRDU antibody |
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10R-2356 | Fitzgerald | 1.5 mL | EUR 490 |
Description: Mouse monoclonal BRDU antibody |
![]() BRDU antibody |
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20-BS17 | Fitzgerald | 250 ug | EUR 386 |
Description: Sheep polyclonal BRDU antibody |
![]() BRDU antibody |
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10R-6763 | Fitzgerald | 1 ml | EUR 402 |
Description: Mouse monoclonal BRDU antibody |
![]() BRDU antibody |
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10R-7945 | Fitzgerald | 100 ug | EUR 370 |
Description: Mouse monoclonal BRDU antibody |
![]() BRDU antibody |
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10R-B121a | Fitzgerald | 100 ug | EUR 445 |
Description: Mouse monoclonal BRDU antibody |
![]() 5-BrdU |
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B3589-1000 | ApexBio | 1 g | EUR 142 |
Description: 5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111. |
![]() 5-BrdU |
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B3589-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 113 |
Description: 5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111. |
![]() 5-BrdU |
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B3589-500 | ApexBio | 500 mg | EUR 108 |
Description: 5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111. |
![]() anti-BrdU |
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BROF-100T | ImmunoStep | 100 test | EUR 537.5 |
![]() 5-BrdU |
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HY-15910 | MedChemExpress | 5g | EUR 497 |
![]() Human BrDU ELISA Kit |
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EHB0693 | Abclonal | 96Tests | EUR 521 |
![]() Mouse BrdU ELISA Kit |
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ELA-E1336m | Lifescience Market | 96 Tests | EUR 865 |
![]() Goat BrDU ELISA Kit |
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EGTB0693 | Abclonal | 96Tests | EUR 521 |
![]() Bovine BrDU ELISA Kit |
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EBB0693 | Abclonal | 96Tests | EUR 521 |
![]() Canine BrDU ELISA Kit |
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ECB0693 | Abclonal | 96Tests | EUR 521 |
![]() Chicken BrDU ELISA Kit |
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ECKB0693 | Abclonal | 96Tests | EUR 521 |
![]() Anserini BrDU ELISA Kit |
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EAB0693 | Abclonal | 96Tests | EUR 521 |
![]() Mouse BrDU ELISA Kit |
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EMB0693 | Abclonal | 96Tests | EUR 521 |
![]() Rat BrDU ELISA Kit |
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ERB0693 | Abclonal | 96Tests | EUR 521 |
![]() Sheep BrDU ELISA Kit |
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ESB0693 | Abclonal | 96Tests | EUR 521 |
![]() Rabbit BrDU ELISA Kit |
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ERTB0693 | Abclonal | 96Tests | EUR 521 |
![]() Monkey BrDU ELISA Kit |
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EMKB0693 | Abclonal | 96Tests | EUR 521 |
![]() Porcine BrDU ELISA Kit |
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EPB0693 | Abclonal | 96Tests | EUR 521 |
![]() apo-B100/ Rat apo- B100 ELISA Kit |
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ELA-E0603r | Lifescience Market | 96 Tests | EUR 886 |
![]() apo-A1/ Rat apo- A1 ELISA Kit |
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ELA-E0604r | Lifescience Market | 96 Tests | EUR 886 |
![]() Apo-E/ Rat Apo- E ELISA Kit |
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ELA-E0704r | Lifescience Market | 96 Tests | EUR 886 |
![]() Apo-E4/ Rat Apo- E4 ELISA Kit |
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ELA-E1858r | Lifescience Market | 96 Tests | EUR 886 |
![]() Human Bromodeoxyuridine,BrdU ELISA Kit |
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201-12-1904 | SunredBio | 96 tests | EUR 440 |
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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
![]() Guinea Pig BrDU ELISA Kit |
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EGB0693 | Abclonal | 96Tests | EUR 521 |
![]() CytoSelect BrdU Competitive ELISA Kit |
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CBA-5098 | Cell Biolabs | 96 assays | EUR 543 |
![]() Rat Bromodeoxyuridine,BrdU ELISA Kit |
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CN-01657R1 | ChemNorm | 96T | EUR 447 |
![]() Rat Bromodeoxyuridine,BrdU ELISA Kit |
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CN-01657R2 | ChemNorm | 48T | EUR 296 |
![]() Mouse Bromodeoxyuridine,BrdU ELISA Kit |
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CN-02530M1 | ChemNorm | 96T | EUR 471 |
![]() Mouse Bromodeoxyuridine,BrdU ELISA Kit |
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CN-02530M2 | ChemNorm | 48T | EUR 322 |
![]() Human Bromodeoxyuridine,BrdU ELISA Kit |
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CN-04581H1 | ChemNorm | 96T | EUR 471 |
![]() Human Bromodeoxyuridine,BrdU ELISA Kit |
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CN-04581H2 | ChemNorm | 48T | EUR 322 |
![]() BrdU Cell Proliferation Assay Kit |
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C0300-050 | GenDepot | 500 Assays | EUR 928 |
![]() BrdU Cell Proliferation Assay Kit |
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C0300-100 | GenDepot | 1000 Assays | EUR 1312 |
![]() BrdU Cell Proliferation Assay Kit |
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C0300-500 | GenDepot | 5000 Assays | EUR 4438 |
![]() Human Bromodeoxyuridine(BrdU)ELISA Kit |
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GA-E1920HM-48T | GenAsia Biotech | 48T | EUR 289 |
![]() Human Bromodeoxyuridine(BrdU)ELISA Kit |
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GA-E1920HM-96T | GenAsia Biotech | 96T | EUR 466 |
![]() Rat Bromodeoxyuridine(BrdU)ELISA Kit |
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GA-E0659RT-48T | GenAsia Biotech | 48T | EUR 317 |
![]() Rat Bromodeoxyuridine(BrdU)ELISA Kit |
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GA-E0659RT-96T | GenAsia Biotech | 96T | EUR 496 |
![]() Mouse Bromodeoxyuridine(BrdU)ELISA Kit |
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GA-E0163MS-48T | GenAsia Biotech | 48T | EUR 336 |
![]() Mouse Bromodeoxyuridine(BrdU)ELISA Kit |
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GA-E0163MS-96T | GenAsia Biotech | 96T | EUR 534 |
![]() BrdU Cell Proliferation Assay Kit |
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K306-1000 | Biovision | EUR 974 |
![]() BrdU Cell Proliferation Assay Kit |
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K306-200 | Biovision | EUR 392 |
![]() Human Bromodeoxyuridine(BrdU)ELISA Kit |
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QY-E00749 | Qayee Biotechnology | 96T | EUR 400 |
![]() Rat Bromodeoxyuridine(BrdU)ELISA Kit |
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QY-E10993 | Qayee Biotechnology | 96T | EUR 361 |
![]() Mouse Bromodeoxyuridine(BrdU)ELISA Kit |
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QY-E20224 | Qayee Biotechnology | 96T | EUR 361 |
![]() BrdU (Bu20a) Antibody |
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48275-100ul | SAB | 100ul | EUR 333 |
![]() BrdU (Bu20a) Antibody |
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48275-50ul | SAB | 50ul | EUR 239 |
![]() Anti-BrdU Antibody |
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A1284-100 | Biovision | EUR 398 |
![]() Anti-BrdU Purified |
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11-286-C025 | ExBio | 0.025 mg | EUR 108 |
![]() Anti-BrdU Purified |
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11-286-C100 | ExBio | 0.1 mg | EUR 177 |
![]() Anti-BrdU Purified |
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11-682-C025 | ExBio | 0.025 mg | EUR 108 |
![]() Anti-BrdU Purified |
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11-682-C100 | ExBio | 0.1 mg | EUR 177 |
![]() Anti-BrdU PE |
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1P-682-T025 | ExBio | 25 tests | EUR 140 |
![]() Anti-BrdU PE |
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1P-682-T100 | ExBio | 100 tests | EUR 240 |
![]() BRDU antibody (FITC) |
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61R-1900 | Fitzgerald | 1 ml | EUR 409 |
Description: Mouse monoclonal BRDU antibody (FITC) |
![]() Bromodeoxyuridine (BrdU) Antibody |
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abx412356-50ug | Abbexa | 50 ug | EUR 509 |
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![]() Bromodeoxyuridine (BrdU) Antibody |
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abx414257-02mg | Abbexa | 0.2 mg | EUR 565 |
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![]() Bromodeoxyuridine (BrdU) Antibody |
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abx414259-01mg | Abbexa | 0.1 mg | EUR 439 |
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![]() Bromodeoxyuridine (BrdU) Antibody |
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abx414260-20ug | Abbexa | 20 ug | EUR 272 |
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![]() Bromodeoxyuridine (BrdU) Antibody |
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abx230952-100ug | Abbexa | 100 ug | EUR 509 |
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![]() BrdU Mouse mAb |
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A1482-100ul | Abclonal | 100 ul | EUR 308 |
![]() BrdU Mouse mAb |
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A1482-200ul | Abclonal | 200 ul | Ask for price |
![]() BrdU Mouse mAb |
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A1482-20ul | Abclonal | 20 ul | Ask for price |
![]() BrdU Mouse mAb |
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A1482-50ul | Abclonal | 50 ul | EUR 223 |
![]() Bromodeoxyuridine (BrdU) Antibody |
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abx000064-100ul | Abbexa | 100 ul | EUR 411 |
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![]() anti- BrdU antibody |
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FNab00952 | FN Test | 100µg | EUR 548.75 |
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Description: Antibody raised against BrdU |
![]() Anti-BrdU antibody |
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STJ16101064 | St John's Laboratory | 100 µg | EUR 354 |
![]() Anti-BrdU antibody |
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STJ22838 | St John's Laboratory | 100 µl | EUR 277 |
![]() STAT Universal Animal IHC KIT-small |
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329ANK-20 | Innovex | Small Kit | EUR 717 |
![]() STAT Universal Animal IHC KIT-large |
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329ANK-60 | Innovex | Large Kit | EUR 1088 |
![]() ApoBrdU-IHC DNA Fragmentation Assay Kit |
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55R-1398 | Fitzgerald | 50 assays | EUR 1086 |
Description: DNA Fragmentation Assay Kit for use in the research laboratory |
![]() ApoBrdU-IHC DNA Fragmentation Assay Kit |
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K2072-50 | ApexBio | 50 assays | EUR 835 |
![]() ApoBrdU-IHC DNA Fragmentation Assay Kit |
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K403-50 | Biovision | EUR 794 |
![]() HiQ Block for IHC |
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B3077-010 | GenDepot | 100ml | EUR 134 |
![]() HiQ Block for IHC |
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B3077-050 | GenDepot | 500ml | EUR 263 |
![]() Frit Kit |
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FRIT-KIT | Next Advance | 1each | EUR 124 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
![]() Column Packing Kit |
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PACK-KIT | Next Advance | 1pack | EUR 1035 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
![]() APO-DIRECTTM Kit (OKTA00007) |
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OKTA00007 | Aviva Systems Biology | KIT | EUR 585 |
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity: |
![]() APO-BrdUTM Kit (OKTA00008) |
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OKTA00008 | Aviva Systems Biology | KIT | EUR 585 |
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity: |
![]() CytoSelect BrdU Cell Proliferation ELISA Kit |
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CBA-251 | Cell Biolabs | 96 assays | EUR 531 |
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation. |
![]() PCR Mycoplasma Detection Kit |
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M034-Kit | TOKU-E | Kit | EUR 266 |
![]() BrdU ELISA Kit| Rat Bromodeoxyuridine ELISA Kit |
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EF018146 | Lifescience Market | 96 Tests | EUR 689 |
![]() Bromodeoxyuridine (BrdU) Antibody (FITC) |
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abx201100-100tests | Abbexa | 100 tests | EUR 620 |
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![]() BrdU (Bu20a) Conjugated Antibody |
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C48275 | SAB | 100ul | EUR 397 |
![]() BrdU (Bromodeoxyuridine)(BRD469) Antibody |
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BNUB0469-100 | Biotium | 100uL | EUR 209 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), Concentration: 0.2mg/mL |
![]() BrdU (Bromodeoxyuridine)(BRD469) Antibody |
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BNUB0469-500 | Biotium | 500uL | EUR 458 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), Concentration: 0.2mg/mL |
![]() BrdU (Bromodeoxyuridine)(BRD494) Antibody |
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BNUB0494-100 | Biotium | 100uL | EUR 209 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD494), Concentration: 0.2mg/mL |
![]() BrdU (Bromodeoxyuridine)(BRD494) Antibody |
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BNUB0494-500 | Biotium | 500uL | EUR 458 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD494), Concentration: 0.2mg/mL |
![]() BrdU (Bromodeoxyuridine)(BU20a) Antibody |
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BNUB0522-100 | Biotium | 100uL | EUR 209 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BU20a), Concentration: 0.2mg/mL |
![]() BrdU (Bromodeoxyuridine)(BU20a) Antibody |
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BNUB0522-500 | Biotium | 500uL | EUR 458 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BU20a), Concentration: 0.2mg/mL |
![]() BrdU (Bromodeoxyuridine)(BRD469) Antibody |
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BNUM0469-50 | Biotium | 50uL | EUR 395 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), 1mg/mL |
![]() BrdU (Bromodeoxyuridine)(BRD494) Antibody |
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BNUM0494-50 | Biotium | 50uL | EUR 395 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD494), 1mg/mL |
![]() BrdU (Bromodeoxyuridine)(BU20a) Antibody |
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BNUM0522-50 | Biotium | 50uL | EUR 395 |
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BU20a), 1mg/mL |
![]() Bromodeoxyuridine (BrdU) Antibody (FITC) |
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abx414258-50ug | Abbexa | 50 ug | EUR 342 |
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![]() Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1110 |
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![]() BCIP/NBT Solution for IHC |
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B3007-005 | GenDepot | 50ml | EUR 134 |
![]() BCIP/NBT Solution for IHC |
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B3007-010 | GenDepot | 100ml | EUR 188 |
![]() BCIP/NBT Solution for IHC |
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B3007-050 | GenDepot | 500ml | EUR 475 |
![]() IHC Enzyme Antigen Retrieval Reagent |
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AR0022 | BosterBio | 50mL (for 500-750 assays) | EUR 106 |
![]() Universal IHC Diluent/Blocker/Stabilizer |
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85R-1021 | Fitzgerald | 1 liter | EUR 692 |
Description: Universal Diluent/Blocker/Stabilizer for use in IHC |
![]() EMA antibody (Prediluted for IHC) |
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75R-1002 | Fitzgerald | 7 ml | EUR 225 |
Description: Mouse monoclonal EMA antibody (Prediluted for IHC) |
![]() hCG antibody (Prediluted for IHC) |
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75R-1005 | Fitzgerald | 7 ml | EUR 244 |
Description: Mouse monoclonal hCG antibody (Prediluted for IHC) |
![]() EpCAM antibody (Prediluted for IHC) |
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75R-1014 | Fitzgerald | 7 ml | EUR 225 |
Description: Mouse monoclonal EpCAM antibody (Prediluted for IHC) |
![]() p53 antibody (Prediluted for IHC) |
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75R-1015 | Fitzgerald | 7 ml | EUR 225 |
Description: Mouse monoclonal p53 antibody (Prediluted for IHC) |
![]() PCNA antibody (Prediluted for IHC) |
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75R-1016 | Fitzgerald | 7 ml | EUR 225 |
Description: Mouse monoclonal PCNA antibody (Prediluted for IHC) |
![]() S100 antibody (Prediluted for IHC) |
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75R-1017 | Fitzgerald | 7 ml | EUR 225 |
Description: Mouse monoclonal S100 antibody (Prediluted for IHC) |
![]() TSH antibody (Prediluted for IHC) |
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75R-1019 | Fitzgerald | 7 ml | EUR 225 |
Description: Mouse monoclonal TSH antibody (Prediluted for IHC) |
![]() Thyroglobulin antibody (Prediluted for IHC) |
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75R-1020 | Fitzgerald | 7 ml | EUR 225 |
Description: Mouse monoclonal Thyroglobulin antibody (Prediluted for IHC) |
![]() NSE antibody (Prediluted for IHC) |
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75R-1023 | Fitzgerald | 7 ml | EUR 232 |
Description: Mouse monoclonal NSE antibody (Prediluted for IHC) |
![]() Ferritin antibody (Prediluted for IHC) |
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75R-1026 | Fitzgerald | 7 ml | EUR 187 |
Description: Rabbit polyclonal Ferritin antibody (Prediluted for IHC) |
![]() Vimmentin antibody (Prediluted for IHC) |
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75R-1027 | Fitzgerald | 7 ml | EUR 187 |
Description: Rabbit polyclonal Vimmentin antibody (Prediluted for IHC) |
![]() Fibronectin antibody (Prediluted for IHC) |
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75R-1028 | Fitzgerald | 7 ml | EUR 187 |
Description: Rabbit polyclonal Fibronectin antibody (Prediluted for IHC) |
![]() CRP antibody (Prediluted for IHC) |
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75R-1029 | Fitzgerald | 7 ml | EUR 187 |
Description: Rabbit polyclonal CRP antibody (Prediluted for IHC) |