A case of mammary myofibroblastoma diagnosed with cytomorphological, cell block and immunohistochemistry findings

A case of mammary myofibroblastoma diagnosed with cytomorphological, cell block and immunohistochemistry findings

The interpretation of outcomes on immunostained cell-block sections needs to be in contrast with the cumulative printed knowledge derived predominantly from formalin-fixed paraffin-embedded (FFPE) tissue sections. Due to this, it is very important acknowledge that the fixation and processing protocol shouldn’t be totally different from the routinely processed FFPE surgical pathology tissue. Publicity to non-formalin fixatives or reagents could intervene with the diagnostic immunoreactivity sample. The immunoprofile noticed on such cell-blocks, which aren’t processed in a fashion just like the surgical pathology specimens, will not be consultant leading to aberrant outcomes.

The sphere of immunohistochemistry (IHC) is advancing constantly with the standardization of many immunomarkers. Quite a lot of technical advances comparable to multiplex IHC with refined methodologies and automation is growing its position in medical purposes. The latest addition of rabbit monoclonal antibodies has additional improved sensitivity. As in comparison with the mouse monoclonal antibodies, the rabbit monoclonal antibodies have 10 to 100 fold greater antigen affinity. Many of the situations contain the analysis of coordinate immunostaining patterns in cell-blocks with comparatively scant diagnostic materials with out correct orientation which is normally retained in a lot of the surgical pathology specimens.

These challenges are addressed if cell-blocks are ready with some devoted methodologies comparable to NextGen CelBloking™ (NGCB) kits. Cell-blocks ready by NGCB kits additionally facilitate the straightforward utility of the SCIP (subtractive coordinate immunoreactivity sample) strategy for correct analysis of coordinate immunoreactivity. Varied cell-block and IHC-related points are mentioned intimately. Pericytes are present in all vascularized organs and are outlined anatomically as perivascular cells that intently encompass endothelial cells in capillaries and microvessels and are embedded inside the similar basement membrane.

mTOR Pathway Activation Assessed by Immunohistochemistry in Cervical Biopsies of HPV-associated Endocervical Adenocarcinomas (HPVA): Correlation With Silva Invasion Patterns

The Silva sample of invasion, not too long ago launched to stratify sufferers in danger for lymph node metastases in human papillomavirus-associated endocervical adenocarcinomas (HPVAs), can solely be assessed in cone and loop electrosurgical excision process excisions with adverse margins or in a hysterectomy specimen. Earlier research discovered associations between harmful stromal invasion patterns (Silva patterns B and C) and mutations in genes concerned within the MEK/PI3K pathways that activate the mammalian goal of rapamycin (mTOR) pathway. The first intention of this research was to make use of cervical biopsies to find out whether or not markers of mTOR pathway activation affiliate with aggressive invasion patterns in matched excision specimens.

The standing of the markers in small biopsy specimens ought to enable us to foretell the ultimate and biologically related sample of invasion in a resection specimen. With the ability to predict the ultimate sample of invasion is necessary, since prediction as Silva A, for instance, would possibly encourage conservative medical administration. If the sample within the resection specimen is B with lymphovascular invasion or C, additional surgical procedure could be carried out 34 HPVA biopsies have been evaluated for expression of pS6, pERK, and HIF1α. Immunohistochemical stains have been scored semiquantitatively, starting from Zero to 4+ with scores 2 to 4+ thought of constructive, and Silva sample was decided in follow-up excisional specimens.

Silva patterns acknowledged in excisional specimens have been distributed as follows: sample A (n=8), sample B (n=4), and sample C (n=22). Statistically vital associations have been discovered evaluating pS6 and pERK immunohistochemistry with Silva sample (P=0.034 and 0.05, respectively). Of the three markers examined, pERK was essentially the most highly effective for distinguishing between sample A and patterns B and C (P=0.026; odds ratio: 6.75, 95% confidence interval: 1.111-41.001). Though the adverse predictive values have been disappointing, the constructive predictive values have been encouraging: 90% for pERK, 88% for pS6 and 100% for HIF1α. mTOR pathway activation assessed by immunohistochemistry in cervical biopsies of HPVA correlate with Silva invasion patterns.

 A case of mammary myofibroblastoma diagnosed with cytomorphological, cell block and immunohistochemistry findings

Detection of BRAF V600E Mutation in Ganglioglioma and Pilocytic Astrocytoma by Immunohistochemistry and Actual-Time PCR-Based mostly Idylla Check

The BRAF V600E mutation is a vital oncological goal in sure central nervous system (CNS) tumors, for which a potential utility of BRAF-targeted remedy grows constantly. Within the current research, we intention to find out the prevalence of BRAF V600E mutations in a sequence of ganglioglioma (GG) and pilocytic astrocytoma (PA) circumstances. Concurrently, we determined to confirm whether or not the mix of absolutely automated tests-BRAF-VE1 immunohistochemistry (IHC) and Idylla BRAF mutation assay-may be helpful to precisely predict it within the case of specified CNS tumors.

The research included 49 formalin-fixed, paraffin-embedded tissues, of which 15 have been GG and 34 PA. Immunohistochemistry with anti-BRAF V600E (VE1) antibody was carried out on tissue sections utilizing the VentanaBenchMark ULTRA platform. All constructive or equivocal circumstances on IHC and chosen adverse ones have been additional assessed utilizing the Idylla BRAF mutation assay coupled with the Idylla platform. The BRAF-VE1 IHC was constructive in 6 (6/49; 12.3%) and adverse in 39 samples (39/49; 79.6%). The interpretation of immunostaining outcomes was sophisticated in Four circumstances, of which 1 examined constructive for the Idylla BRAF mutation assay. Subsequently, the general positivity fee was 14.3%.

Streptavidin

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Streptavidin

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Description: The best epigenetics products

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FNSA-0001 500 uL
EUR 324
Description: Streptavidin secondary antibody

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Streptavidin Fluorescent Particle Kit

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EUR 462
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Streptavidin Fluorescent Particle Kit

SVFA-2558--6K 6x1 mL
EUR 462
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Streptavidin Fluorescent Particle Kit

SVFB-2552--6K 6x1 mL
EUR 462
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Streptavidin Fluorescent Particle Kit

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Streptavidin, Streptavidin Recombinant Protein, His Tag

PROTP22629-1 Regular: 20ug
EUR 317
Description: Recombinant Streptomyces Avidinii Streptavidin produced in E.Coli is a single, non-glycosylated polypeptide chain (25-183) containing a total of 167 amino acids and having a molecular mass of 17kDa. The Streptavidin protein is fused to an 8 aa N-terminal His-Tag and purified by proprietary chromatographic techniques.

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
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CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

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Streptavidin/HRP

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EUR 257
Description: Streptavidin/HRP by Cygnus Technologies is available in Europe via Gentaur.

Streptavidin -HRP

E61I01602H 100ug
EUR 343

Streptavidin Protein

abx061011-1mg 1 mg
EUR 286

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abx020705-1mg 1 mg
EUR 648

Recombinant Streptavidin

7-06553 5mg Ask for price

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7-06555 1gr Ask for price

Streptavidin Protein

20-abx261434
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Streptavidin Protein

abx261783-1gr 1 gr
EUR 4128

Streptavidin Protein

20-abx261783
  • EUR 328.00
  • EUR 230.00
  • 20 mg
  • 5 mg

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  • EUR 1344.00
  • EUR 328.00
  • EUR 230.00
  • 100 mg
  • 10 mg
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Streptavidin Antibody

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  • EUR 411.00
  • EUR 1845.00
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  • 100 ug
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Streptavidin Antibody

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  • EUR 411.00
  • EUR 1845.00
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  • 100 ug
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Streptavidin, Lyophilized

S0819-002 2mg
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S0819-010 10mg
EUR 415

Streptavidin Polystyrene

SVP-1000-4 4 mL
EUR 472
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Streptavidin Antibody

21155-05011 150 ug
EUR 217

Streptavidin protein

PROTP22629 Regular: 10mg
EUR 317
Description: Streptavidin is a protein produced by Streptomyces avidinii and isolated by purification from fermentation broth. The pure, homogeneous protein shows predominantly one single band in SDS PAGE. Streptavidin consists of 4 identical subunits, each bearing an active binding site for biotin. Streptavidin has a molecular weight of 55kDa.

Streptavidin, CF514

29081 1mg
EUR 253
Description: Minimum order quantity: 1 unit of 1mg

Streptavidin (APC)

abx670347-100tests 100 tests
EUR 398

Streptavidin (FITC)

abx670348-1mg 1 mg
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abx670349-1ml 1 ml
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abx670350-1mg 1 mg
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abx670351-1mg 1 mg
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abx670352-1mg 1 mg
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Streptavidin Antibody

1-CSB-PA18194A0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Streptavidin. Recognizes Streptavidin from Streptomyces avidinii. This antibody is Unconjugated. Tested in the following application: ELISA

Streptavidin Antibody

1-CSB-PA332849YA01SNO
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Streptavidin. Recognizes Streptavidin from Streptomyces avidinii. This antibody is Unconjugated. Tested in the following application: ELISA

Core Streptavidin

7936-1
EUR 142

Core Streptavidin

7936-10
EUR 528

Core Streptavidin

7936-5
EUR 316

Streptavidin - FITC

P1498-10 10 mg
EUR 171

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P1498-50 50 mg
EUR 530

Streptavidin antibody

20R-3017 2 mL
EUR 503
Description: Rabbit polyclonal Streptavidin antibody

Streptavidin antibody

10R-1103 100 ul
EUR 316
Description: Mouse monoclonal Streptavidin antibody

Streptavidin antibody

10R-S118a 1 mg
EUR 489
Description: Mouse monoclonal Streptavidin antibody

Streptavidin antibody

10R-S118b 1 mg
EUR 457
Description: Mouse monoclonal Streptavidin antibody

Streptavidin antibody

10R-S118c 1 mg
EUR 392
Description: Mouse monoclonal Streptavidin antibody

Streptavidin protein

30C-CE0301 10 mg
EUR 547
Description: Purified homogeneous preparation of Streptavidin protein

Streptavidin (PE)

80R-2357 250 ug
EUR 165
Description: Purified Streptavidin conjugated to Phycoerythrin

Streptavidin (Cy3)

XG-6187Cy3 0.5 mg
EUR 424.1
Description: Streptavidin (Cy3)

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XG-6187F 0.5 mg
EUR 424.1
Description: Streptavidin (FITC)

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XG-6187HRP 0.5 mg
EUR 424.1
Description: Streptavidin (HRP)

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132

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EUR 1132

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Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

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EUR 1132

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EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

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Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153

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PIN320A-KIT 1 Kit
EUR 4941

AP-streptavidin conjugate [Streptavidin-alkaline phosphatase conjugate]

16921 1 mg
EUR 176

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941

Streptavidin Blue Array Particle Kit

SVPAK-5067--10K 10 mL
EUR 994
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Streptavidin Blue Array Particle Kit

SVPAK-5067--5A 5X1 mL
EUR 588
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Streptavidin Blue Array Particle Kit

SVPAK-5067--5B 5X1 mL
EUR 588
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
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BA1088-0.5 0.5ml
EUR 146

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BA1088-1 1ml
EUR 233

Streptavidin Magnetic Beads

HY-K0208 5 mL
EUR 538

ProMag HP Streptavidin

PMS3-HP-1 1 ml
EUR 221.4
Description: ProMag HP Streptavidinsuitable for use in assay developmen. This item is supplied as suspension in water.

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PMS3HP-10 10 ml
EUR 929.36
Description: ProMag HP Streptavidinsuitable for use in assay developmen. This item is supplied as suspension in water.

This included 2 circumstances of GG and 5 circumstances of PA. Our research discovered that BRAF V600E mutations are reasonably frequent in PA and GG and that for these tumor entities, IHC VE1 is appropriate for screening functions, however all adverse, equivocal, and weak constructive circumstances ought to be additional examined with molecular biology methods, of which the Idylla system appears to be a promising device. They’ve been proven to have various physiological and pathological capabilities together with regulation of blood stress, and tissue regeneration and scarring. Basic to understanding the position these cells play in these various processes is the flexibility to precisely establish and localize them in vivo. To do that, we’ve developed multicolor immunohistochemistry protocols described on this chapter.

Harmonization across programmed death ligand 1 (PD-L1) assays for lung cancer by immunohistochemistry using noncontact alternating current electric field mixing

Harmonization across programmed death ligand 1 (PD-L1) assays for lung cancer by immunohistochemistry using noncontact alternating current electric field mixing
Immune checkpoint inhibitors (ICIs) are a promising advance within the therapy of sufferers with lung most cancers. Nonetheless, every ICI has been examined with an independently designed companion diagnostic assay that’s primarily based on a singular antibody. Consequently, the completely different trial-validated programmed demise ligand 1 (PD-L1) immunohistochemistry (IHC) assays shouldn’t be thought of interchangeable. Our intention was to check the efficiency of every out there PD-L1 antibody for its skill to precisely measure PD-L1 expression and to analyze the potential of harmonization throughout antibodies by means of using a brand new speedy IHC system, which makes use of noncontact alternating present (AC) mixing to attain extra secure staining.
First, 58 resected non-small cell lung most cancers (NSCLC) specimens had been stained utilizing three PD-L1 IHC assays (28-8, SP142, and SP263) to evaluate the harmonization achieved with AC mixing IHC. Second, specimens from 27 sufferers receiving ICIs for postoperative recurrent NSCLC had been stained utilizing the identical IHC technique to check the scientific efficiency of ICIs to PD-L1 scores. All sufferers obtained a tumor proportion rating (TPS) with the 22C3 companion diagnostic check.
Higher staining was achieved with the brand new AC mixing IHC technique than the traditional IHC in PD-L1-positive instances, and the interchangeability of some combos of assays was elevated in PD-L1-positive. As well as, AC mixing IHC offered extra acceptable general response charges for ICIs in all assays. Sixteen out of 85 tumors confirmed constructive staining representing 5% of FB tumors, 24% of CCS tumors and 47% of FM. In FB and CCS tumors, constructive staining was primarily encountered in atypical intraepidermal melanocytic proliferations and spitzoid neoplasms. The specificity of constructive PRAME staining was 95% and its concordance with the ultimate diagnostic interpretation was 75%.

Impression of Preanalytical Components on the Measurement of Tumor Tissue Biomarkers Utilizing Immunohistochemistry

Evaluation of formalin-fixed paraffin-embedded (FFPE) tissue by immunohistochemistry (IHC) is commonplace in scientific and analysis laboratories. Nonetheless, reviews recommend that IHC outcomes might be compromised by biospecimen preanalytical elements. The Nationwide Most cancers Institute’s Biospecimen Preanalytical Variables Program performed a scientific research to look at the potential results of delay to fixation (DTF) and time in fixative (TIF) on IHC utilizing 24 most cancers biomarkers. Variations in IHC staining, relative to controls with a DTF of 1 hr, had been noticed in FFPE kidney tumor specimens after a DTF of ≥2 hr.

Reductions in H-score and/or staining depth had been noticed for c-MET, p53, PAX2, PAX8, pAKT, and survivin, whereas will increase had been noticed for RCC1, EGFR, and CD10. Extended TIF of 72 hr resulted in considerably lowered H-scores of CD44 and c-Met in kidney tumor specimens, in contrast with controls with 12-hr TIF. An elevated likelihood of altered staining depth attributable to DTF was noticed for 9 antigens, whereas for extended TIF an elevated likelihood was noticed for one antigen.

Outcomes reported right here and elsewhere throughout tumor sorts and antigens assist limiting DTF to ≤1 hr when attainable and fixing tissues in formalin for 12-24 hr to keep away from confounding results of those preanalytical elements on IHC. The persistent an infection of high-risk human papillomavirus (HR-HPV) is among the most typical causes of cervical most cancers worldwide, and HPV kind 58 (HPV58) is the third most typical HPV kind in japanese Asia. The E7 oncoprotein is constitutively expressed in HPV58-associated cervical most cancers cells and performs a key function throughout tumorigenesis. This research aimed to evaluate the HPV58 E7 protein expression within the tissues of cervical most cancers and cervical intraepithelial neoplasia (CIN).

 Harmonization across programmed death ligand 1 (PD-L1) assays for lung cancer by immunohistochemistry using noncontact alternating current electric field mixing

NKX3.1 immunohistochemistry is very particular for the prognosis of mesenchymal chondrosarcomas: expertise within the Australian inhabitants

Mesenchymal chondrosarcoma (MC) is a uncommon sarcoma that usually arises in adolescents and younger adults and characteristically harbours a HEY1-NCOA2 gene fusion. A current research has proven that NKX3.1 immunohistochemistry (IHC) is very particular and delicate in MCs. NKX3.1 is a nuclear marker expressed in prostatic tissue and is broadly utilized in most laboratories to find out prostatic origin of metastatic tumours. Within the present research we investigated whether or not this stain can be utilized within the diagnostic workup of MC, as it could help in triaging instances for additional molecular testing, by assessing its expression in a cohort of MCs and in a large spectrum of sarcoma sorts.
Moreover, we aimed to elucidate if expression of NKX3.1 by MCs is expounded to androgen receptor (AR) expression. We recognized NKX3.1 constructive nuclear staining in 9 of 12 particular person sufferers of MC (n=20 of 25 samples when taking into consideration separate episodes). 4 of the 5 unfavorable specimens had been beforehand subjected to acid-based decalcification. NKX3.1 was unfavorable in 536 samples from 16 non-MC sarcomas derived from largely tissue microarrays (TMAs). Total, we recognized 80% sensitivity and 100% specificity for NKX3.1 IHC in MCs.

Immunohistochemistry Kit (OKRA00038)

OKRA00038 1 kit
EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Immunohistochemistry Kit (OKRA00039)

OKRA00039 1 kit
EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Immunohistochemistry Kit (OKRA00040)

OKRA00040 1 kit
EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

BrdU/ Rat BrdU ELISA Kit

ELA-E1336r 96 Tests
EUR 886

Brdu

20-abx082348
  • EUR 189.00
  • EUR 300.00
  • 100 mg
  • 1 g

5-BrdU

HY-15910 5g
EUR 497

5-BrdU

B3589-1000 1 g
EUR 142
Description: 5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.

5-BrdU

B3589-5.1 10 mM (in 1mL DMSO)
EUR 113
Description: 5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.

5-BrdU

B3589-500 500 mg
EUR 108
Description: 5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.

anti-BrdU

BROF-100T 100 test
EUR 537.5

BRDU antibody

20R-BS001 250 ug
EUR 553
Description: Sheep polyclonal BRDU antibody

BRDU antibody

10R-6763 1 ml
EUR 402
Description: Mouse monoclonal BRDU antibody

BRDU antibody

10R-7945 100 ug
EUR 370
Description: Mouse monoclonal BRDU antibody

BRDU antibody

10R-B121a 100 ug
EUR 445
Description: Mouse monoclonal BRDU antibody

BRDU antibody

20-BS17 250 ug
EUR 386
Description: Sheep polyclonal BRDU antibody

BRDU antibody

10R-2321 1 mL
EUR 601
Description: Mouse monoclonal BRDU antibody

BRDU antibody

10R-2356 1.5 mL
EUR 490
Description: Mouse monoclonal BRDU antibody

Sheep BrDU ELISA Kit

ESB0693 96Tests
EUR 521

Mouse BrDU ELISA Kit

EMB0693 96Tests
EUR 521

Anserini BrDU ELISA Kit

EAB0693 96Tests
EUR 521

Rabbit BrDU ELISA Kit

ERTB0693 96Tests
EUR 521

Monkey BrDU ELISA Kit

EMKB0693 96Tests
EUR 521

Porcine BrDU ELISA Kit

EPB0693 96Tests
EUR 521

Rat BrDU ELISA Kit

ERB0693 96Tests
EUR 521

Human BrDU ELISA Kit

EHB0693 96Tests
EUR 521

Canine BrDU ELISA Kit

ECB0693 96Tests
EUR 521

Chicken BrDU ELISA Kit

ECKB0693 96Tests
EUR 521

Goat BrDU ELISA Kit

EGTB0693 96Tests
EUR 521

Bovine BrDU ELISA Kit

EBB0693 96Tests
EUR 521

Mouse BrdU ELISA Kit

ELA-E1336m 96 Tests
EUR 865

apo-B100/ Rat apo- B100 ELISA Kit

ELA-E0603r 96 Tests
EUR 886

apo-A1/ Rat apo- A1 ELISA Kit

ELA-E0604r 96 Tests
EUR 886

Apo-E/ Rat Apo- E ELISA Kit

ELA-E0704r 96 Tests
EUR 886

Apo-E4/ Rat Apo- E4 ELISA Kit

ELA-E1858r 96 Tests
EUR 886

Mouse Bromodeoxyuridine(BrdU)ELISA Kit  

GA-E0163MS-48T 48T
EUR 336

Mouse Bromodeoxyuridine(BrdU)ELISA Kit  

GA-E0163MS-96T 96T
EUR 534

Rat Bromodeoxyuridine(BrdU)ELISA Kit

GA-E0659RT-48T 48T
EUR 317

Rat Bromodeoxyuridine(BrdU)ELISA Kit

GA-E0659RT-96T 96T
EUR 496

Human Bromodeoxyuridine(BrdU)ELISA Kit

GA-E1920HM-48T 48T
EUR 289

Human Bromodeoxyuridine(BrdU)ELISA Kit

GA-E1920HM-96T 96T
EUR 466

Guinea Pig BrDU ELISA Kit

EGB0693 96Tests
EUR 521

BrdU Cell Proliferation Assay Kit

C0300-050 500 Assays
EUR 928

BrdU Cell Proliferation Assay Kit

C0300-100 1000 Assays
EUR 1312

BrdU Cell Proliferation Assay Kit

C0300-500 5000 Assays
EUR 4438

Human Bromodeoxyuridine,BrdU ELISA Kit

201-12-1904 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

CytoSelect BrdU Competitive ELISA Kit

CBA-5098 96 assays
EUR 543

Rat Bromodeoxyuridine(BrdU)ELISA Kit

QY-E10993 96T
EUR 361

Mouse Bromodeoxyuridine(BrdU)ELISA Kit

QY-E20224 96T
EUR 361

Human Bromodeoxyuridine(BrdU)ELISA Kit

QY-E00749 96T
EUR 400

Mouse Bromodeoxyuridine,BrdU ELISA Kit

CN-02530M1 96T
EUR 471

Mouse Bromodeoxyuridine,BrdU ELISA Kit

CN-02530M2 48T
EUR 322

Human Bromodeoxyuridine,BrdU ELISA Kit

CN-04581H1 96T
EUR 471

Human Bromodeoxyuridine,BrdU ELISA Kit

CN-04581H2 48T
EUR 322

Rat Bromodeoxyuridine,BrdU ELISA Kit

CN-01657R1 96T
EUR 447

Rat Bromodeoxyuridine,BrdU ELISA Kit

CN-01657R2 48T
EUR 296

BrdU Cell Proliferation Assay Kit

K306-1000
EUR 974

BrdU Cell Proliferation Assay Kit

K306-200
EUR 392

Anti-BrdU antibody

STJ22838 100 µl
EUR 277

Anti-BrdU antibody

STJ16101064 100 µg
EUR 354

Anti-BrdU Purified

11-286-C025 0.025 mg
EUR 108

Anti-BrdU Purified

11-286-C100 0.1 mg
EUR 177

Anti-BrdU Purified

11-682-C025 0.025 mg
EUR 108

Anti-BrdU Purified

11-682-C100 0.1 mg
EUR 177

Anti-BrdU PE

1P-682-T025 25 tests
EUR 140

Anti-BrdU PE

1P-682-T100 100 tests
EUR 240

Bromodeoxyuridine (BrdU) Antibody

abx000064-100ul 100 ul
EUR 411

BrdU Mouse mAb

A1482-100ul 100 ul
EUR 308

BrdU Mouse mAb

A1482-200ul 200 ul Ask for price

BrdU Mouse mAb

A1482-20ul 20 ul Ask for price

BrdU Mouse mAb

A1482-50ul 50 ul
EUR 223

Bromodeoxyuridine (BrdU) Antibody

abx412356-50ug 50 ug
EUR 509

Bromodeoxyuridine (BrdU) Antibody

abx414257-02mg 0.2 mg
EUR 565

Bromodeoxyuridine (BrdU) Antibody

abx414259-01mg 0.1 mg
EUR 439

Bromodeoxyuridine (BrdU) Antibody

abx414260-20ug 20 ug
EUR 272

Bromodeoxyuridine (BrdU) Antibody

abx230952-100ug 100 ug
EUR 509

anti- BrdU antibody

FNab00952 100µg
EUR 548.75
Description: Antibody raised against BrdU

BrdU (Bu20a) Antibody

48275-100ul 100ul
EUR 333

BrdU (Bu20a) Antibody

48275-50ul 50ul
EUR 239

Anti-BrdU Antibody

A1284-100
EUR 398

BRDU antibody (FITC)

61R-1900 1 ml
EUR 409
Description: Mouse monoclonal BRDU antibody (FITC)

STAT Universal Animal IHC KIT-small

329ANK-20 Small Kit
EUR 717

STAT Universal Animal IHC KIT-large

329ANK-60 Large Kit
EUR 1088

ApoBrdU-IHC DNA Fragmentation Assay Kit

K2072-50 50 assays
EUR 835

ApoBrdU-IHC DNA Fragmentation Assay Kit

K403-50
EUR 794

ApoBrdU-IHC DNA Fragmentation Assay Kit

55R-1398 50 assays
EUR 1086
Description: DNA Fragmentation Assay Kit for use in the research laboratory

HiQ Block for IHC

B3077-010 100ml
EUR 134

HiQ Block for IHC

B3077-050 500ml
EUR 263

APO-DIRECTTM Kit (OKTA00007)

OKTA00007 KIT
EUR 585
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

APO-BrdUTM Kit (OKTA00008)

OKTA00008 KIT
EUR 585
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

CytoSelect BrdU Cell Proliferation ELISA Kit

CBA-251 96 assays
EUR 531
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

BrdU ELISA Kit| Rat Bromodeoxyuridine ELISA Kit

EF018146 96 Tests
EUR 689

BrdU (Bromodeoxyuridine)(BRD469) Antibody

BNUM0469-50 50uL
EUR 395
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), 1mg/mL

BrdU (Bromodeoxyuridine)(BRD494) Antibody

BNUM0494-50 50uL
EUR 395
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD494), 1mg/mL

BrdU (Bromodeoxyuridine)(BU20a) Antibody

BNUM0522-50 50uL
EUR 395
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BU20a), 1mg/mL

BrdU (Bromodeoxyuridine)(BRD469) Antibody

BNUB0469-100 100uL
EUR 209
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), Concentration: 0.2mg/mL

BrdU (Bromodeoxyuridine)(BRD469) Antibody

BNUB0469-500 500uL
EUR 458
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD469), Concentration: 0.2mg/mL

BrdU (Bromodeoxyuridine)(BRD494) Antibody

BNUB0494-100 100uL
EUR 209
Description: Primary antibody against BrdU (Bromodeoxyuridine)(BRD494), Concentration: 0.2mg/mL
The sensitivity elevated to 95.2% when acid-based decalcified specimens had been excluded from the evaluation. No correlation between NKX3.1 expression and AR IHC was recognized. In abstract, our findings point out that NKX3.1 nuclear positivity is very delicate and particular for MC, offered that ethylenediaminetetraacetic acid (EDTA)-based quite than acid-based decalcification is used for pattern processing. NKX3.1 IHC in the precise scientific and histopathological setting can doubtlessly be ample for the prognosis of MC, reserving molecular affirmation just for equivocal instances.

Predictive “Biomarker Piggybacking”: An Examination of Reflexive Pan-Cancer Screening with Pan-TRK Immunohistochemistry

Predictive "Biomarker Piggybacking": An Examination of Reflexive Pan-Cancer Screening with Pan-TRK Immunohistochemistry
ropomyosin receptor kinase (TRK) focused therapies symbolize an necessary therapeutic possibility for sufferers with superior stable tumors harboring neurotrophin receptor kinase (NTRK) gene fusions. Nevertheless, NTRK fusions are uncommon in frequent grownup carcinomas, and systematic approaches to screening for these alterations are missing. Pan-TRK immunohistochemistry (IHC) has been proposed as one methodology to display screen for NTRK fusion-positive tumors. Reflexive testing methods have been endorsed for a number of IHC-based biomarkers, and thus supply a handy and low-cost entry level to include pan-TRK screening.
 On this examine, 447 consecutive circumstances of grownup stable tumors present process mismatch restore (MMR), HER2, and/or PD-L1 testing have been prospectively stained with pan-TRK IHC. 4 circumstances (0.9%) have been pan-TRK optimistic, together with 3 (1.3% of 223) colonic adenocarcinomas, of which 2 have been MMR-deficient, and one (1.4% of 71) gastroesophageal carcinoma. None of 108 non-small cell lung carcinomas confirmed pan-TRK expression. NTRK gene fusion was confirmed by DNA sequencing in a single MMR-deficient colonic adenocarcinoma. In a single MMR-deficient tumor, an alternate MAPK driver was recognized.
Within the esophageal (squamous cell) carcinoma, RNA sequencing recognized relative NTRK2 transcript overexpression within the absence of a fusion. In a single MMR-proficient colonic adenocarcinoma, no MAPK drivers had been recognized; subsequently, a falsely damaging sequencing end result was favored. Not one of the sufferers met medical standards for TRK focused remedy. Immunohistochemistry (IHC) allows the selective detection of proteins in cells of formalin-fixed-paraffin-embedded (FFPE) tissue sections. This method performs a key position within the identification and classification of main lung most cancers tumors by means of the analysis of the expression of the aspartic proteinase Napsin-A.
Nevertheless, immunohistochemistry is a posh course of involving many important steps and the dearth of standardization in addition to inappropriate analytical circumstances might contribute to inconsistent outcomes between laboratories. Automated immunohistochemistry addresses this subject by making certain the standard and the reproducibility of the outcomes amongst totally different laboratories. Right here we describe an automatic IHC protocol utilized in our laboratory for the detection of Napsin-A in FFPE lung tissue sections.

Detection of Programmed Cell Dying Ligand 1 Expression in Lung Most cancers Scientific Samples by an Automated Immunohistochemistry System

Programmed cell demise 1 (PD-1) performs an necessary position in subsiding immune responses, in selling self-tolerance by means of suppressing the exercise of T-cells, and in selling differentiation of regulatory T-cells. One in every of its ligands, programmed cell demise ligand 1 (PD-L1) acts as a checkpoint regulator in immune cells and can also be expressed in a variety of most cancers varieties. Anti-PD remedy modulates immune responses on the tumor web site, targets tumor-induced immune defects, and repairs ongoing immune responses.
Since medicine that focus on the PD-1/PD-L1 pathways grew to become obtainable as a most cancers remedy, there may be want for the usage of totally different antibodies to detect the presence of those proteins in tumoral samples by immunohistochemistry or different assays. As a result of the detection of those antigens in tumor samples is extremely clinically informative for guiding remedy choices, particularly to determine the aptness of a affected person to obtain anti-PD remedy Because of this, it’s important to outline and choose the most effective antibody clones and validate them utilizing totally different strategies with the intention to have a dependable detection of optimistic staining when these antibodies are utilized in IHC.
it’s essential to have a validation course of that guaranties that the check outcomes obtained when utilizing antibodies in opposition to these proteins are particular, selective, reproducible, and conducive to quantification of antigen abundance in most cancers tissue sections. Right here we describe an automatic immunohistochemistry staining process that may be utilized for the validation of a number of anti-PD-L1 antibody clones when used for the staining of formalin-fixed, paraffin-embedded lung most cancers tissue sections.

Predictive "Biomarker Piggybacking": An Examination of Reflexive Pan-Cancer Screening with Pan-TRK ImmunohistochemistryAutomated TTF-1 Immunohistochemistry Assay for the Differentiation of Lung Adenocarcinoma Versus Lung Squamous Cell Carcinoma

Because of therapeutic advances, the subclassification of non-small cell lung carcinomas (NSCLC) between the adenocarcinomas and squamous cell carcinomas subtypes is important for the observe of personalised and focused drugs. The medical administration for these two NSCLC subtypes is totally different because of their totally different molecular properties and histological origins. Immunohistochemistry (IHC) markers such is TTF-1 play a key position within the differentiation of lung adenocarcinomas and squamous cell carcinomas. Nevertheless, immunohistochemistry is a posh course of involving many important steps and the reliability of outcomes will depend on the standardization of the assay in addition to the suitable interpretation.
Completely different laboratories use totally different reagents and totally different IHC approaches for the detection of TTF-1 in lung most cancers tumors. Right here we describe an automatic IHC protocol utilized in our laboratory for the detection of TTF-1 in formalin-fixed, paraffin-embedded (FFPE) tissue sections from lung tumors. Antibody choice and optimization are essential to ensure correct and reproducible outcomes when utilizing such antibodies for functions resembling western blot evaluation and immunohistochemistry (IHC). That is particularly necessary when deciding on good candidate antibodies that will likely be used for most cancers immunotherapy diagnostics and analysis.

DAB Chromogen/Substrate Kit

ACH500 500 Slides
EUR 85

DAB Substrate (High Contrast)

ACU250 250 ml
EUR 85

DAB Substrate (High Contrast)

ACU500 500 ml
EUR 98

DAB Substrate (High Contrast)

ACU999 1000 ml
EUR 119

DAB Chromogenic Substrate Kit (Brown)

AR1022 1 kit (for 600-900 assays)
EUR 80

DAB Chromogenic Substrate Kit (Blue)

AR1025 1 kit (for 600-900 assays)
EUR 80

DAB Chromogen/Substrate Bulk pack

ACK500 530 ml
EUR 120

DAB Chromogen/Substrate Bulk pack

ACK999 1060 ml
EUR 176

STAT-Q Mouse Adsorbed DAB KIT; for staining Rat antibodies-on-Mouse tissues 250 plus slide kit

NB314MAK-DAB 200-250 slides
EUR 856

STAT-Q Rat Adsorbed DAB Kit: for staining Mouse antibodies-on-Rat tissues 250 plus slides kit

NB314RAK-DAB 200-250 slides
EUR 856

DAB Chromogen/Substrate Kit (High Contrast)

ACT500 500 Slides
EUR 157

3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs

CH027 50 Tabs
EUR 177

3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs

CH028 100 Tabs
EUR 233

Immunohistochemistry Kit (OKRA00037)

OKRA00037 1 kit
EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Immunohistochemistry Kit (OKRA00038)

OKRA00038 1 kit
EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Immunohistochemistry Kit (OKRA00039)

OKRA00039 1 kit
EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

Immunohistochemistry Kit (OKRA00040)

OKRA00040 1 kit
EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV250 265 ml
EUR 125

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV500 530 ml
EUR 165

DAB Chromogen/Substrate Bulk Pack (High Contrast)

ACV999 1060 ml
EUR 236

DAB Liquid/Substrate (stable), 2-component system, 70ml

NB314SBD 70 ml
EUR 392

DAB

HY-15912 500mg
EUR 108

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 30ml

NB319-SBB 30 ml
EUR 369

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04727 5g Ask for price

Fmoc-Dab[Fmoc-Dab(Boc)]-OH

5-04728 25g Ask for price

DAB Tablet

D3225-020 20TB
EUR 142

DAB Tablet

D3225-050 50TB
EUR 246

Black-DAB Substrate/Chromogen System for IHC-HRP staining (stable), 70ml NEW

NB319-BB70 70 ml
EUR 485

DAB Chromogen Concentrate

D3226-002 20ml
EUR 161

DAB Chromogen Concentrate

D3226-005 50ml
EUR 334

Boc-Dab-OH

A-3215.0001 1.0g
EUR 126
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0005 5.0g
EUR 418
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0025 25.0g
EUR 1578
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

H-Dab-OH·HCl

5-04703 5g Ask for price

H-Dab-OH·HCl

5-04704 25g Ask for price

Boc-Dab-OH

5-04711 5g Ask for price

Boc-Dab-OH

5-04712 25g Ask for price

Fmoc-Dab-OAll·HCl

5-04720 5g Ask for price

Fmoc-Dab-OH·HCl

5-04721 5g Ask for price

Fmoc-Dab-OH·HCl

5-04722 25g Ask for price

Fmoc-Dab-OH

5-04735 1g Ask for price

Fmoc-Dab-OH

5-04736 5g Ask for price

DAB Chromogen Kit

abx097197-1Kit 1 Kit
EUR 272

H-Dab-OH

F-3050.0001 1.0g
EUR 115
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0005 5.0g
EUR 225
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0025 25.0g
EUR 803
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

Z-Dab-OH

C-3705.0001 1.0g
EUR 115
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0005 5.0g
EUR 273
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0025 25.0g
EUR 998
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Fmoc-Dab-OH

B-2300.0001 1.0g
EUR 151
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

Fmoc-Dab-OH

B-2300.0005 5.0g
EUR 515
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

DAB Chromogen, 4ml

NB314D 4 ml
EUR 246

DL-Dab.2HCl

A6727-100000 100 g
EUR 569
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-25000 25 g
EUR 309
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-5000 5 g
EUR 180
Description: DL-Dab.2HCl

DAB Chromogen Concentrate

ACB030 30 ml
EUR 119

DAB Chromogen Concentrate

ACB060 60 ml
EUR 165

DAB Chromogen Concentrate

ACB125 125 ml
EUR 245

DAB Chromogen Concentrate

ACB500 500 ml
EUR 671

DAB Chromogen Concentrate

ACB999 1000 ml
EUR 1159

DAB Enhancer Solution

ACM030 30 ml
EUR 71

DAB Away Kit

ACP001 500 ml
EUR 98

DAB Differentiating Solution

DDS500 500 ml
EUR 109

DAB Enhancer for ampliying DAB stains in IHC staining, 60ml

NB308 60 ml
EUR 427

DAB Enhancer for ampliying DAB stains in IHC staining, 30ml

NB308-30 30 ml
EUR 299

Sensitive DAB Stain Kit

PW023 5Preps, 5prep
EUR 69.14

H-Dab(Boc)-OH

A-3305.0001 1.0g
EUR 200
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

H-Dab(Boc)-OH

A-3305.0005 5.0g
EUR 696
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

Boc-Dab(Fmoc)-OH

A-3520.0001 1.0g
EUR 225
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Fmoc)-OH

A-3520.0005 5.0g
EUR 780
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Aloc)-OH

A-4125.0001 1.0g
EUR 309
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-Dab(Aloc)-OH

A-4125.0005 5.0g
EUR 1143
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-D-Dab-OH

A-4215.0001 1.0g
EUR 418
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-D-Dab-OH

A-4215.0005 5.0g
EUR 1554
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-Dab-OtBu · HCl

A-4415.0001 1.0g
EUR 309
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Boc-Dab-OtBu · HCl

A-4415.0005 5.0g
EUR 1143
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

H-Dab(Z)-OH

5-04707 5g Ask for price

H-Dab(Z)-OH

5-04708 25g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04713 1g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04714 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04715 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04716 25g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04717 5g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04718 25g Ask for price

Fmoc-Dab(Ac)-OH

5-04723 5g Ask for price

Fmoc-Dab(Ac)-OH

5-04724 25g Ask for price

Fmoc-Dab(Boc)-OH

5-04725 5g Ask for price

Fmoc-Dab(Boc)-OH

5-04726 25g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04729 1g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04730 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04731 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04732 25g Ask for price

Fmoc-Dab(Mtt)-OH

5-04733 1g Ask for price

Fmoc-Dab(Mtt)-OH

5-04734 5g Ask for price

Fmoc-Dab(Dde)-OH

5-04739 1g Ask for price

Fmoc-Dab(Dde)-OH

5-04740 5g Ask for price
On this chapter, we describe a Western Blot approach as help methodology for the choice and validation of Programmed Cell Dying Ligand 1 (PD-L1) antibodies that may be subsequently utilized in immunohistochemistry functions. Western Blot is a delicate, particular, and broadly obtainable protein characterization approach, used for the detection of particular antigens. PD-L1 is a significant immune checkpoint protein that mediates antitumor immune suppression and response, which is routinely detected utilizing IHC in formalin-fixed and paraffin-embedded tissues as a part of most cancers medical diagnostic workflows.